![]() Introduction Researches confirmed good antioxidant activity in fruit peels. It could be concluded that feeding of orange peel wastes to animals might be beneficial due to presence of good amount of beta carotene in it. The result showed presence of significant (P<0.05) amount of beta carotene in orange peels followed by mango peel and pomegranate peel. Beta carotene was extracted by ethanol: hexane (4:3, v/v) solvent and the extracts were subjected for HPTLC analysis. The present study plans to detect and quantify beta carotenes in fruit peel wastes collected from a beverage production center in Kolkata. The allied techniques, classic and modern, demonstrated different ways of evaluating the essential oil through its chemical composition, both techniques showed high efficiency and precision, in addition was an appropriate project developed by postgraduate students.īeta carotene is an important molecule that is precursor to vitamin A and possesses multiple health benefits. monandra flower showed a predominance of 70.22% of sesquiterpenic compounds. In the GC-MS analysis, 7 compounds were observed, being two majorities, characterized as panaxene with 20.51% and the α-guaiene with 33.39%. In the TLC analysis 18 Rfs were observed after the use of different developers, with the predominant class of oxygenates compounds. The essential oil of the flowers showed a yield of 0.06%, positive solubility in ethanol 70%, refractive index of 1.3621, optical rotation of +36.4αD and relative density of 0.941 g mL-1 at 20 ☌. Essential oil was extracted by Clevenger, the TLC was performed in different eluents and developers, and thus the retention factors (Rfs), and the chemical profile by GC-MS were obtained. This paper investigate a characterization by thin layer chromatography (TLC) and gas chromatography coupled with mass spectrometry (GC-MS), as a method developed by graduate students that involve a combination of a classic and modern technique, as well as results about the physicochemical properties of the essential oil of Bauhinia monandra flower. The study of chromatographic techniques, classical and modern, describes the simplicity and, at the same time, the advances that this area has undergone in recent years in quality scientific research and also in learning in undergraduate and postgraduate courses around the world. ![]()
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